Coding

Part:BBa_K4623000:Design

Designed by: Yixin Huang   Group: iGEM23_BNU-China   (2023-10-08)


Silica-binding peptide (SBP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Among various SBPs, we have chosen SBP5 for use in our project.This modification greatly enhances the binding capacity of SBP5. Furthermore, compared to other SBPs, SBP5 exhibits binding affinity within an intermediate range, avoiding excessive binding strength that would render it insensitive to solution.

Source

We got the sequence through gene synthesis.SBP is derived from Car9 and the modification involves changing the orientation of the imidazole plane and introducing a positively charged histidine-rich region to mitigate the negative charge effects.


References

[1]Chang Liu, David L. Steer, Haipeng Song, and Lizhong He The Journal of Physical Chemistry Letters 2022 13 (6), 1609-1616 DOI: 10.1021/acs.jpclett.1c03306.